The quality of control tests of H2O2 decontamination processes strongly depends on the used microorganism. Therefore, biological indicators are always certified with an underlying strain.
Since biological indicators are to be used without a special containment facility, bacterial spores are required that retain their resistance properties over the specified shelf life. Bacterial spores of Geobacillus species are therefore usually used for biological indicators.
The quality control team of SKAN Laboratories provides appropriate test methods to control the microorganisms used for biological indicators and to document their use based on recognized standards.
- How many biological indicators are used for a microbiological identification?
According to our SKAN test procedure, 3 biological indicators are usually required as the basis for a microbiological identification.
- How is the microbiological identification performed?
The identification is performed by DNA sequencing of a 500 bp-long fragment of the 16S rDNA and subsequent genotypic identification of the microorganism on species level. The obtained sequences are finally compared with 16S rDNA Library to generate an identity match. For biological indicators, samples are processed by SKAN laboratories before being sequenced externally.