{"id":12366,"date":"2021-09-06T15:05:47","date_gmt":"2021-09-06T15:05:47","guid":{"rendered":"https:\/\/skan.clients.ideeal.ch\/?page_id=12366"},"modified":"2025-07-08T07:09:54","modified_gmt":"2025-07-08T07:09:54","slug":"d-value-estimation","status":"publish","type":"page","link":"https:\/\/skan.com\/en\/services\/quality-control\/d-value-estimation\/","title":{"rendered":"D-Value Estimation"},"content":{"rendered":"\t<!-- section hero-->\r\n\t<section id=\"section-hero-block_e18ae542db4a6d9919f510ffdb145954\" class=\"section-hero full-height-screen viewport-section\" >\r\n\t\t\t\t\t<a class=\"link-page-back cusomt-mod\" href=\"https:\/\/skan.com\/en\/services\/quality-control\/\" target=\"_self\">\r\n\t\t\t\t<span class=\"page-name\">Quality Control<\/span>\r\n\t\t\t<\/a>\r\n\t\t\t\t<div class=\"container-fluid\">\r\n\t\t\t<div class=\"full-width-row full-height-holder df-row\">\r\n\t\t\t\t\t\t\t\t\t<div class=\"column-text col-lg-6\">\r\n\t\t\t\t\t\t<div class=\"banner-text text-lead text-secondary js-anim-delay-parent-500 js-anim-default-parent\">\r\n\t\t\t\t\t\t\t<h1>D-Value Estimation<\/h1>\n<p>Quality control testing\u00a0for biological indicators<\/p>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"scroll-down-block d-lg-block\">\r\n\t\t\t\t\t\t\t\t<a class=\"link-anchor link-anchor-down\" href=\"#anchor-block\">\r\n\t\t\t\t\t\t\t\t\tScroll down\t\t\t\t\t\t\t\t<\/a>\r\n\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t<\/div>\r\n\t\t\t\t\t            \t\t\t\t\t<div class=\"column-image col-lg-6\">\r\n\t\t\t\t\t\t                                                            <div class=\"visual-box animImage\">\r\n                                                            <picture>\r\n                                                                            <source type=\"image\/jpeg\" srcset=\"https:\/\/skan.com\/wp-content\/uploads\/skan-services-quality-control-d-value-estimation.jpg 375w, https:\/\/skan.com\/wp-content\/uploads\/skan-services-quality-control-d-value-estimation.jpg 750w\" sizes=\"100vw\" media=\"(max-width: 767px)\"\/>\r\n                                                                        <source type=\"image\/jpeg\" srcset=\"https:\/\/skan.com\/wp-content\/uploads\/skan-services-quality-control-d-value-estimation.jpg 736w, https:\/\/skan.com\/wp-content\/uploads\/skan-services-quality-control-d-value-estimation.jpg 1472w\" sizes=\"(min-width: 768px) 50vw, 100vw\"\/>\r\n                                    <img loading=\"lazy\" decoding=\"async\" width=\"1472\" height=\"2000\" src=\"https:\/\/skan.com\/wp-content\/uploads\/skan-services-quality-control-d-value-estimation.jpg\" class=\"attachment-full size-full\" alt=\"Qualit\u00e4tssicherungspr\u00fcfung f\u00fcr biologische Indikatoren\" \/>                                <\/picture>\r\n                            <\/div>\t\t\t\t\t\t                        \t\t\t\t\t<\/div>\r\n\t            \t\t\t<\/div>\r\n\t\t<\/div>\r\n\t<\/section>\r\n\t<div id=\"anchor-block\"><\/div>\r\n\n\n\t<section id=\"section-content-block_d23a6e8360e4f78d9e1449690db90b3c\" class=\"section-content info-section-2-columns\">\r\n\t\t<div class=\"container-fluid\">\r\n\t\t\t<div class=\"two-blocks-content\">\r\n\t\t\t\t\t\t\t\t\t<div class=\"article-wrap\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"block heading-block\">\r\n\t\t\t\t\t\t\t\t<p>The D-Value, or decimal reduction time, represents the time required to inactivate 90 % of a given microorganism under particular conditions. If less biological indicators are tested than the D-Value determination requires, the result is a reasonable estimate that can be used as a basis for quality control of a batch of biological indicators.<\/p>\n\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"block\">\r\n\t\t\t\t\t\t\t\t<p>The quality control team of SKAN Laboratories offers a D-Value estimation based on recognized standards. Testing takes place exclusively in SKANs H<sub>2<\/sub>O<sub>2<\/sub> decontamination facility to simulate real conditions.<\/p>\n\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"block\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h2>FAQs<\/h2>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<ul class=\"accordion accordion-content\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<li>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"opener\">How many biological indicators are used for a D-Value Estimation?<\/a>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"slide\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"slide-body\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<p>According to the SKAN test procedure, 31 biological indicators are required to perform a valid D-Value estimation. Since this procedure is an approximation, more accurate results can be obtained if more biological indicators are used.<\/p>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/li>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<li>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"opener\">Which media are used to control the inactivation state?<\/a>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"slide\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"slide-body\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<p>After successfully completing the H<sub>2<\/sub>O<sub>2<\/sub> decontamination of the isolator system, the biological indicators are incubated in tubes containing 9 mL of Trypcase Soy Broth (TSB). Biological indicators that are not completely inactivated will cloud the carrier liquid. Statistical analysis of the tubes showing active and inactive biological indicators provides the estimate of the D-Value.<\/p>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/li>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/ul>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t<\/div>\r\n\t<\/section>\r\n\n\n\t<!-- section services-->\r\n\t<section id=\"section-services-block_339399a8ae2cc19318608759c20830fd\" class=\"section-main section-services \">\r\n\t\t<div class=\"container-fluid\">\r\n\t\t\t\t\t\t\t<h2 class=\"title-section\">Other services<\/h2>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t<!-- gallery services list-->\r\n\t\t\t\t<div class=\"gallery-services-list slider-with-controls-mobile\">\r\n\t\t\t\t\t<div class=\"controls\">\r\n\t\t\t\t\t\t<!--.dots-->\r\n\t\t\t\t\t\t<div class=\"arrows\"><\/div>\r\n\t\t\t\t\t<\/div>\r\n\t\t\t\t\t<div class=\"list-slider-mobile\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"slide-service\">\r\n\t\t\t\t\t\t\t\t<div class=\"slide-holder popup-service-wrap\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"card-service open-popup-service\" href=\"#\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"card-img\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"96\" height=\"96\" src=\"https:\/\/skan.com\/wp-content\/uploads\/Mikrobiologische-Bestimmung.svg\" class=\"attachment-full size-full\" alt=\"\" \/>\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"card-body\">\r\n\t\t\t\t\t\t\t\t\t\t\t<h3 class=\"card-title\">Microbiological Identification<\/h3>\r\n\t\t\t\t\t\t\t\t\t\t\t<p>Confirmation of the bacterial species on biological indicators<\/p>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"link-wrap\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"link-arrowed\">Details<span class=\"plus\"><\/span><\/span>\r\n\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t<\/a>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"popup-service\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"close-popup-service btn btn-secondary btn-close\" href=\"#\">Close<\/a>\r\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"popup-service-holder\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"card-service-popup \">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"card-img\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"96\" height=\"96\" src=\"https:\/\/skan.com\/wp-content\/uploads\/Mikrobiologische-Bestimmung.svg\" class=\"attachment-full size-full\" alt=\"\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"card-body\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"title-block\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h2 class=\"card-title\">Microbiological Identification<\/h2>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h3 class=\"card-subtitle\">Confirmation of the bacterial species on biological indicators.<\/h3>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<p>The quality of control tests of H<sub>2<\/sub>O<sub>2<\/sub> decontamination processes strongly depends on the used microorganism. Therefore, biological indicators are always certified with an underlying strain.\r\n<br><br>\r\nSince biological indicators are to be used without a special containment facility, bacterial spores are required that retain their resistance properties over the specified shelf life. Bacterial spores of Geobacillus species are therefore usually used for biological indicators. \r\n<br><br>\r\nThe quality control team of SKAN Laboratories provides appropriate test methods to control the microorganisms used for biological indicators and to document their use based on recognized standards.<\/p>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"contact-box\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h3>Interested in learning more?<\/h3>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"btn-wrap\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"btn btn-secondary\" href=\"https:\/\/skan.com\/en\/services\/quality-control\/microbiological-identification\/\" target=\"_self\">To the service<\/a>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"slide-service\">\r\n\t\t\t\t\t\t\t\t<div class=\"slide-holder popup-service-wrap\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"card-service open-popup-service\" href=\"#\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"card-img\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"96\" height=\"96\" src=\"https:\/\/skan.com\/wp-content\/uploads\/Total-Kill-Test.svg\" class=\"attachment-full size-full\" alt=\"\" \/>\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"card-body\">\r\n\t\t\t\t\t\t\t\t\t\t\t<h3 class=\"card-title\">Total Kill Test<\/h3>\r\n\t\t\t\t\t\t\t\t\t\t\t<p>Confirmation of biological indicator\u2019s resistance homogeneity<\/p>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"link-wrap\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"link-arrowed\">Details<span class=\"plus\"><\/span><\/span>\r\n\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t<\/a>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"popup-service\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"close-popup-service btn btn-secondary btn-close\" href=\"#\">Close<\/a>\r\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"popup-service-holder\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"card-service-popup \">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"card-img\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"96\" height=\"96\" src=\"https:\/\/skan.com\/wp-content\/uploads\/Total-Kill-Test.svg\" class=\"attachment-full size-full\" alt=\"\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"card-body\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"title-block\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h2 class=\"card-title\">Total Kill Test<\/h2>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h3 class=\"card-subtitle\">Confirmation of biological indicator\u2019s resistance homogeneity.<\/h3>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<p>In order to reliably answer the question about the quality of a decontamination based on H<sub>2<\/sub>O<sub>2<\/sub>, the batch of the biological indicator must be homogeneous with respect to existing resistances.  For the test, the biological indicators are placed in the plant or isolator and the well-known decontamination procedure is performed as standard. The indicators are then analyzed to determine whether they are inactivated. The degree of inactivation can be derived as the quality of decontamination.\r\n<br><br>\r\nThe SKAN Laboratories quality control team performs the Total Kill Test based on recognized standards with high quality documentation using a reference SKAN H<sub>2<\/sub>O<sub>2<\/sub> decontamination system.\r\n<\/p>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"contact-box\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h3>Interested in learning more?<\/h3>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"btn-wrap\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"btn btn-secondary\" href=\"https:\/\/skan.com\/en\/services\/quality-control\/total-kill-test\/\" target=\"_self\">To the service<\/a>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"slide-service\">\r\n\t\t\t\t\t\t\t\t<div class=\"slide-holder popup-service-wrap\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"card-service open-popup-service\" href=\"#\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"card-img\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"96\" height=\"96\" src=\"https:\/\/skan.com\/wp-content\/uploads\/D-Value-Determination.svg\" class=\"attachment-full size-full\" alt=\"\" \/>\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"card-body\">\r\n\t\t\t\t\t\t\t\t\t\t\t<h3 class=\"card-title\">D-Value Determination<\/h3>\r\n\t\t\t\t\t\t\t\t\t\t\t<p>Determination of the spore\u2019s resistance on biological indicators<\/p>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"link-wrap\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"link-arrowed\">Details<span class=\"plus\"><\/span><\/span>\r\n\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t<\/a>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"popup-service\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"close-popup-service btn btn-secondary btn-close\" href=\"#\">Close<\/a>\r\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"popup-service-holder\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"card-service-popup \">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"card-img\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"96\" height=\"96\" src=\"https:\/\/skan.com\/wp-content\/uploads\/D-Value-Determination.svg\" class=\"attachment-full size-full\" alt=\"\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"card-body\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"title-block\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h2 class=\"card-title\">D-Value Determination<\/h2>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h3 class=\"card-subtitle\">Determination of the spore\u2019s resistance on biological indicators<\/h3>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<p>The decimal reduction time describes the amount of time required to kill 90 % of a specific microorganism. This time period is referred to as the D-Value. The determination of this D-Value is a mandatory requirement for the quality control of batches of biological indicators. The D-Value determination determines the informative value of the biological indicators, which are subsequently used to verify the H<sub>2<\/sub>O<sub>2<\/sub>-based decontamination. \r\n<br><br>\r\nThe SKAN Laboratories team is experienced in the determination of the D-Value and performs all control procedures based on recognized standards.<\/p>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"contact-box\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h3>Interested in learning more?<\/h3>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"btn-wrap\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"btn btn-secondary\" href=\"https:\/\/skan.com\/en\/services\/quality-control\/d-value-determination\/\" target=\"_self\">To the service<\/a>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"slide-service\">\r\n\t\t\t\t\t\t\t\t<div class=\"slide-holder popup-service-wrap\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"card-service open-popup-service\" href=\"#\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"card-img\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"96\" height=\"96\" src=\"https:\/\/skan.com\/wp-content\/uploads\/Mikrobiologisches-Monotoring.svg\" class=\"attachment-full size-full\" alt=\"\" \/>\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"card-body\">\r\n\t\t\t\t\t\t\t\t\t\t\t<h3 class=\"card-title\">Viable Spore Count (VSC)<\/h3>\r\n\t\t\t\t\t\t\t\t\t\t\t<p>Evaluation of the spore population on biological indicators<\/p>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"link-wrap\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"link-arrowed\">Details<span class=\"plus\"><\/span><\/span>\r\n\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t<\/a>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"popup-service\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"close-popup-service btn btn-secondary btn-close\" href=\"#\">Close<\/a>\r\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"popup-service-holder\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"card-service-popup \">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"card-img\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"96\" height=\"96\" src=\"https:\/\/skan.com\/wp-content\/uploads\/Mikrobiologisches-Monotoring.svg\" class=\"attachment-full size-full\" alt=\"\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"card-body\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"title-block\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h2 class=\"card-title\">Viable Spore Count (VSC)<\/h2>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h3 class=\"card-subtitle\">Evaluation of the spore population on biological indicators<\/h3>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<p>In order to be able to determine the quality of decontamination tests, SKAN has developed a method for counting viable, isolated spores. The method is based on the scientific principle that spores are capable of germination and growth on solid culture media, and that each microbial colony-forming unit (CFU) represents a direct progeny of a single spore in the original inoculum.\r\n<br><br>\r\nThis is done by serial dilution of the spore in a suitable medium after resuspension from the carrier. The final dilution is then plated out on a suitable medium. After the spores have grown into colonies on this medium, they are counted. The Viable Spore Count results correspond to the amount of Geobacillus stearothermophilus spores that were initially inoculated on the biological indicator.<\/p>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"contact-box\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h3>Interested in learning more?<\/h3>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"btn-wrap\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"btn btn-secondary\" href=\"https:\/\/skan.com\/en\/services\/quality-control\/viable-spore-count-vsc\/\" target=\"_self\">To the service<\/a>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t<\/div>\r\n\t\t\t\t\t<\/div>\r\n\t<\/section>\r\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":5,"featured_media":0,"parent":12456,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":""},"class_list":["post-12366","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.9 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>D-Value Estimation - SKAN<\/title>\n<meta name=\"description\" content=\"The D-Value, or decimal reduction time, represents the time required to inactivate 90 % of a given microorganism under particular conditions. 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